make this paragraph shorther:Next-generation sequencing (NGS): This is a more recent method that revolutionized the field of DNA sequencing. It encompasses a range of high-throughput sequencing technologies, including pyrosequencing, Illumina sequencing, and Ion Torrent sequencing, among others. NGS methods involve massively parallel sequencing of millions of DNA fragments simultaneously. In general, the DNA sample is fragmented into small pieces, attached to adapters that enable clonal amplification, and then amplified through various methods. These amplified fragments are sequenced in parallel using nucleotide incorporation and detection techniques, with each nucleotide incorporation emitting a fluorescence or light signal that can be detected and recorded by a computer. The sequences of these millions of fragments are then aligned and assembled to reconstruct the original DNA sequence. NGS allows for rapid and cost-effective sequencing of large genomes and is widely used in various fields, including genomics, transcriptomics, and metagenomics.
NGS is a recent method that revolutionized DNA sequencing. It involves high-throughput sequencing technologies like pyrosequencing, Illumina sequencing, and Ion Torrent sequencing. NGS methods fragment the DNA sample, amplify it, and sequence the fragments simultaneously. Each nucleotide incorporation emits a fluorescence or light signal that a computer detects and records. The sequences are then aligned and assembled to reconstruct the original DNA sequence. NGS is widely used in genomics, transcriptomics, and metagenomics for rapid and cost-effective sequencing.
