make this paragraph shorther: Sanger sequencing: Frederick Sanger invented this technique in 1977. It is sometimes referred to as dideoxy sequencing or chain termination sequencing. In a reaction mixture containing a tiny number of dideoxynucleotides—which lack the 3' hydroxyl group required for further DNA strand elongation—new DNA strands are synthesised utilising DNA polymerase. A collection of DNA fragments with varying lengths that are all terminated with the same nucleotide are produced by the process. By examining the sizes of the separated fragments from smallest to biggest, the sequence of these fragments may be inferred. These fragments are separated using gel electrophoresis according to their length.
Sanger sequencing, also called dideoxy or chain termination sequencing, was invented by Frederick Sanger in 1977. It involves synthesizing new DNA strands using DNA polymerase in a reaction mixture with dideoxynucleotides, which lack the necessary 3' hydroxyl group for further elongation. This process produces a collection of DNA fragments with varying lengths, all terminated with the same nucleotide. To determine the sequence, the fragments are separated by length using gel electrophoresis.
