How will you prepare 500 ml of 5 percent dextrose solution?
To prepare 500 ml of a 5 percent dextrose solution, you will need the following:
1. Dextrose powder: Dextrose is a type of sugar that can be easily dissolved in water. It is commonly available in powder form. You will need 5 percent of dextrose, which means 5 grams per 100 ml of solution.
2. Distilled or sterile water: You will need water that is free from impurities, so it's recommended to use distilled or sterile water to ensure the solution is clean.
Now, let's proceed with the steps:
1. Calculate the amount of dextrose needed: To prepare a 5 percent dextrose solution, you need 5 grams of dextrose per 100 ml of solution. Since you need 500 ml of solution, you would calculate it as follows:
- 5 grams/100 ml * 500 ml = 25 grams of dextrose.
2. Measure out the required amount of dextrose: Using a scale, measure out 25 grams of dextrose powder.
3. Dissolve the dextrose in water: Take a clean container, such as a beaker or flask, and add approximately 400-450 ml of distilled or sterile water into it. Slowly add the measured dextrose powder into the water, while stirring continuously. This will help the dextrose dissolve completely.
4. Adjust the volume: Once the dextrose is dissolved, add more distilled or sterile water to bring the total volume to 500 ml. Ensure the solution is well-mixed.
5. Label and store the solution: Once the solution is prepared, it is important to label it as a 5 percent dextrose solution with the date of preparation. Store it in a clean, sealed container at the appropriate temperature as per the requirements.
Remember to always follow proper aseptic techniques and handle equipment and materials with care to prevent contamination.