Question

1. a) prepare 1M, 50ml stock solution of Tris HCl

b) from the stock solution, prepare 24mM, 100ml solution

Answer 1

a. How many moles do you want? That's M x L = ?

weigh out grams = mols x molar mass = ? , place in a 50 mL volumetric flask, dissolve, make to the mark on the flask, mix thoroughly and stopper.
b. mL stock x M stock = mLsoln x M soln.
mL stock x 1 = 100 mL x 0.024 M
Solve for mL stock soln, pipet that amount into a 100 mL volumetric flask, etc.
Post your work if you get stuck.