Which of the following was most responsible for the rise of the two-party system in the U.S. government?

• the argument over whether or not the colonies should declare independence
• the bitter conflict over slavery
• the competing views over the adoption of the U.S. Constitution
• the treatment of Native Americans

the competing views over the adoption of the U.S. Constitution

The competing views over the adoption of the U.S. Constitution, specifically the debates between the Federalists and Anti-Federalists, played a significant role in the rise of the two-party system in the U.S. government. The disagreements over the structure and powers of the federal government led to the formation of political parties with differing ideologies and policies. This eventually evolved into the modern two-party system of Democrats and Republicans.