5. Give the steps of an experiment to prove that photosynthesis occurs with both light dependent and light independent phases.
1. Set up two separate groups of plants under identical conditions with one group being exposed to light and the other group kept in darkness.
2. Allow the plants to undergo photosynthesis for a period of time, ensuring that all other environmental factors remain constant.
3. After the allotted time, harvest leaves from both groups of plants.
4. Extract the pigments from the leaves and analyze them using chromatography to determine the presence of chlorophyll and other pigments.
5. Measure the oxygen production in both groups using a gas exchange chamber to determine the rate of photosynthesis.
6. Compare the results between the two groups to see if there is a noticeable difference in pigments and oxygen production, indicating the presence of both light-dependent and light-independent phases of photosynthesis.