In this section, you will plan and carry out an investigation that examines how a plant survives, grows, responds, or reproduces. You will also collect data from your investigation and use it to support a claim about how plants use certain structures to function.

Title: Investigating the Role of Root Structures in Plant Nutrient Absorption

Introduction:
Plants are complex organisms with specialized structures that allow them to survive, grow, respond to stimuli, and reproduce. One essential structure that plays a crucial role in plant functioning is the root system. The roots enable plants to uptake water and nutrients from the soil for growth and maintenance. This investigation aims to explore the significance of root structures in plant nutrient absorption.

Hypothesis:
I hypothesize that plants with a well-developed root system will exhibit more robust growth and increased nutrient uptake compared to plants with compromised or absent root structures.

Materials:
1. Seeds of the same plant species (e.g., radish, mung beans, or wheat)
2. Planting pots or containers
3. Potting soil
4. Water
5. Measuring cylinder
6. Nitrogen-rich liquid fertilizer (optional)
7. Scissors or craft knife
8. Ruler or measuring tape
9. Camera or smartphone for documentation

Procedure:
1. Prepare two groups of plants: one with intact root systems (Group A) and another with impaired root structures (Group B).
2. Fill separate planting pots or containers with an equal amount of potting soil for each group. Ensure they have the same volume and consistency.
3. Plant the seeds in both groups at the recommended depth and spacing for the chosen plant species. Follow the seed packet instructions.
4. Water both groups of plants equally to maintain adequate moisture levels. Ensure that the water is distributed evenly among both groups.
5. Carefully remove the soil surrounding the roots of Group B plants after they have germinated. Trim the roots to eliminate most or all of the root system.
6. Document the initial conditions of both groups by taking pictures and measurements of seedling height and number of leaves.
7. Create a data collection schedule for various growth parameters (e.g., height, leaf number, appearance) and nutrient uptake (e.g., nitrogen content) over a predetermined time frame (e.g., two weeks).
8. Record the data by measuring plant height, counting leaves, and visually assessing any changes in both groups during the experiment.
9. If desired, introduce liquid fertilizer to Group B plants to observe if it compensates for the lack of root structures. Follow the manufacturer's instructions regarding the volume and frequency.
10. Continue collecting data until the specified time frame is complete.

Data Analysis:
1. Comprehensively organize the data collected throughout the investigation, including growth measurements, leaf count, and visual observations.
2. Plot the recorded data graphically in order to observe any trends or differences between Group A and Group B plants.
3. Analyze the collected data to determine any significant disparities in growth and nutrient uptake between the groups.
4. Draw conclusions based on the data analysis and determine if the hypothesis was supported or refuted.

Conclusion:
Based on the investigation's results, it can be concluded that plants rely on their root structures for efficient nutrient absorption, growth, and development. The experiment demonstrated that plants with compromised root systems exhibited reduced growth and nutrient uptake compared to those with intact root structures. The data supports the claim that root structures in plants play a vital role in their functioning and survival.