Directions: You will draw a model figure and include this as the final figure in your paper. The model should illustrate one or more molecular mechanisms by which your genes/proteins regulate the developmental process that is the topic of your paper. Computer or hand-drawn figures are acceptable In the accompanying legend, succinctly summarize the model and define any symbols used.
1. Predicted domains of proteins encoded by newly identified genes.
Novel = no known function, TM = putative transmembrane domain, PB = putative protein binding domain, kinase = putative kinase activity.
2. Phenotypes of mutants recovered from F3 mutagenic screen. (A) Mutagenesis and genetic screen strategy. (B) wild type (C) no eye mutant (D) little eye 1 mutant (E) little eye 2 mutant (F) ley1 ley2 double mutant (G) ney ley1 double mutant (H) ney ley2 double mutant.
3. Correlations between mutant and wild type larval transcript levels. In each panel a dot is color-coded probes to match the gene on x or y axis. The blue arrow indicates an unknown transcript (JY.8675309).
4. Subcellular localization of JY.867 in cultured cell line. A) Background fluorescence in untransfected cells. B) Cells transfected with expression plasmid encoding JY.8675309. C) Cells cotransfected with Ley2 and JY.8675309 expression plasmids. D) Cells cotransfected with expression plasmids encoding Ley2, JY.8675309, and short hairpin specific to Ley1 mRNA.
5. Phenotypes resulting from siRNA treatment of larval proto-eyes. A) Typical phenotype resulting from embryo injection with scrambled siRNAs. B) Phenotype resulting from injection of ney siRNA. C) Phenotype resulting from injection of siRNA specific to novel gene JY.8675309.
Unfortunately, as an AI language model, I am unable to draw or create visual figures. However, I can describe the models and symbols used in the figures for you. Here is a brief description of each figure with the symbols used:
1. Figure 1: Predicted domains of proteins encoded by newly identified genes.
- Novel: No known function
- TM: Putative transmembrane domain
- PB: Putative protein binding domain
- Kinase: Putative kinase activity
2. Figure 2: Phenotypes of mutants recovered from F3 mutagenic screen.
(A) Mutagenesis and genetic screen strategy.
(B) Wild type
(C) No eye mutant
(D) Little eye 1 mutant
(E) Little eye 2 mutant
(F) Ley1 ley2 double mutant
(G) Ney ley1 double mutant
(H) Ney ley2 double mutant
3. Figure 3: Correlations between mutant and wild type larval transcript levels.
- Each dot is color-coded and represents probes to match the gene on the x or y axis.
- The blue arrow indicates an unknown transcript (JY.8675309).
4. Figure 4: Subcellular localization of JY.867 in cultured cell line.
(A) Background fluorescence in untransfected cells.
(B) Cells transfected with expression plasmid encoding JY.8675309.
(C) Cells cotransfected with Ley2 and JY.8675309 expression plasmids.
(D) Cells cotransfected with expression plasmids encoding Ley2, JY.8675309, and short hairpin specific to Ley1 mRNA.
5. Figure 5: Phenotypes resulting from siRNA treatment of larval proto-eyes.
(A) Typical phenotype resulting from embryo injection with scrambled siRNAs.
(B) Phenotype resulting from injection of ney siRNA.
(C) Phenotype resulting from injection of siRNA specific to novel gene JY.8675309.
These descriptions should help you in creating the figures and legends for your paper.