Laboratory Diagnosis of genus pasteurella
The laboratory diagnosis of genus Pasteurella involves several steps to accurately identify and confirm the presence of these bacteria. Here is a general overview of the laboratory procedures involved:
1. Sample Collection: A clinical sample, such as blood, pus, or tissue, is collected from the suspected infection site. The sample should be collected using a sterile technique to avoid contamination.
2. Sample Processing: The collected sample is processed in the laboratory to isolate and culture the Pasteurella bacteria. This involves streaking the sample onto selective agar plates, such as blood agar or MacConkey agar, which promote the growth of Pasteurella bacteria while inhibiting the growth of other organisms.
3. Phenotypic Identification: Once the bacteria have grown on the agar plates, they are subjected to various biochemical tests to identify their phenotypic characteristics. These tests can include Gram staining, catalase test, oxidase test, and other specific tests for Pasteurella species.
4. Antibiotic Sensitivity Testing: The isolated Pasteurella bacteria can be tested for their antibiotic sensitivity to guide appropriate treatment. This is typically done using the Kirby-Bauer disk diffusion method, where different antibiotic disks are placed on the agar plates and the zone of inhibition around each disk is measured to determine antibiotic susceptibility.
5. Molecular Identification: In some cases, molecular techniques such as Polymerase Chain Reaction (PCR) may be used to identify and confirm the presence of Pasteurella bacteria. This technique can detect specific DNA sequences or genes that are characteristic of the genus Pasteurella.
It is important to note that laboratory diagnosis may vary depending on the specific species of Pasteurella being investigated. Some species, such as Pasteurella multocida, are more commonly associated with infections in humans and animals and, therefore, may have specific diagnostic tests available. Consulting a microbiologist or infectious disease specialist is recommended for accurate diagnosis and appropriate treatment.