Which of the following mutations would be most likely to have a harmful effect on an organism?

Evolution of three bases near the center of a gene.
There is not enough information to know whether a mutation would be harmful.
All mutations have a harmful effect on organisms.
A substitution of a thymine four adenine.

A substitution of a thymine for adenine would be most likely to have a harmful effect on an organism. This is because substitutions can alter the genetic code, leading to changes in the amino acid sequence of a protein. This can affect the proper functioning of the protein, potentially causing a harmful effect on the organism.