Follow directions on this station’s card to create complementary DNA strands.
16. AGT-GTG-CAC-ACT
17. GCT-CTC-AGA-GAT
16. TCA-CAC-GTG-TGA
17. CGA-GAG-TCT-CTA
Explain
To create complementary DNA strands, we need to match each nucleotide base with its complement. In DNA, the four nucleotide bases are adenine (A), thymine (T), cytosine (C), and guanine (G).
For the first given sequence, "AGT-GTG-CAC-ACT," we can match each base with its complement as follows:
A - T
G - C
T - A
G - C
C - G
A - T
C - G
T - A
So, the complementary DNA strand for "AGT-GTG-CAC-ACT" is "TCA-CAC-GTG-TGA."
Similarly, for the second given sequence, "GCT-CTC-AGA-GAT," we can match each base with its complement as follows:
G - C
C - G
T - A
C - G
A - T
G - C
A - T
T - A
So, the complementary DNA strand for "GCT-CTC-AGA-GAT" is "CGA-GAG-TCT-CTA."
To create complementary DNA strands, you will need to follow the base-pairing rules of DNA. In DNA, adenine (A) always pairs with thymine (T), and cytosine (C) always pairs with guanine (G).
Let's start with the first sequence, "AGT-GTG-CAC-ACT" (sequence 16). To create the complementary DNA strand, you will need to replace each base with its complementary base.
A (adenine) pairs with T (thymine)
G (guanine) pairs with C (cytosine)
T (thymine) pairs with A (adenine)
G (guanine) pairs with C (cytosine)
C (cytosine) pairs with G (guanine)
A (adenine) pairs with T (thymine)
C (cytosine) pairs with G (guanine)
A (adenine) pairs with T (thymine)
T (thymine) pairs with A (adenine)
So the complementary DNA strand for "AGT-GTG-CAC-ACT" (sequence 16) is "TCA-CAC-GTG-TGA".
Now let's move on to the second sequence, "GCT-CTC-AGA-GAT" (sequence 17).
G (guanine) pairs with C (cytosine)
C (cytosine) pairs with G (guanine)
T (thymine) pairs with A (adenine)
C (cytosine) pairs with G (guanine)
A (adenine) pairs with T (thymine)
G (guanine) pairs with C (cytosine)
A (adenine) pairs with T (thymine)
T (thymine) pairs with A (adenine)
So the complementary DNA strand for "GCT-CTC-AGA-GAT" (sequence 17) is "CGA-GAG-TCT-CTA".
Remember, the process of creating a complementary DNA strand requires replacing each base with its complementary base according to the base-pairing rules.