what is a restriction site?

A restriction site is a specific DNA sequence that is recognized and cut by a restriction enzyme. These enzymes act like molecular scissors that cut the DNA at specific points, usually resulting in a staggered cut that leaves single-stranded overhangs called sticky ends. Restriction sites are used in genetic engineering to insert or remove genes from DNA sequences, among other applications.

A restriction site, also known as a recognition site, is a specific sequence of DNA where a restriction enzyme can bind and cut the DNA molecule. These sites are typically palindromic, meaning the sequence reads the same on both strands when read in opposite directions. Restriction enzymes are proteins that recognize specific DNA sequences and cleave the DNA at or near these sites. These enzymes are essential tools in molecular biology techniques such as DNA cloning and genetic engineering.