A restriction site is a specific DNA sequence that is recognized and cut by a restriction enzyme. These sites are usually palindromic, meaning the sequence reads the same backwards and forwards. When the restriction enzyme recognizes and cuts at the restriction site, it creates fragments of DNA that can be separated and analyzed by gel electrophoresis. Restriction sites are commonly used in molecular biology techniques such as gene cloning and mapping.
A restriction site, also known as a recognition site, is a specific sequence of DNA where a restriction enzyme can bind and cut the DNA molecule. Restriction enzymes are enzymes that recognize specific DNA sequences and cut the DNA at or near those sequences. These sites are typically palindromic, meaning they read the same forward and backward on opposite DNA strands. The cuts made by restriction enzymes are generally staggered, resulting in "sticky ends" or "blunt ends" depending on the type of enzyme used. These cut ends can then be used in molecular biology techniques such as DNA cloning, recombinant DNA technology, and gene editing.
