In cell fractionation. the purpose of keeping the tissue sample in an isotonic solution in a refrigerator prior to homogenization is?
The purpose of keeping the tissue sample in an isotonic solution in a refrigerator prior to homogenization in cell fractionation is to maintain the structural integrity and physiological conditions of the cells.
Cell fractionation is a technique used to separate and isolate different cellular components for further study or analysis. By subjecting the tissue sample to various physical and biochemical methods, such as homogenization, differential centrifugation, and density gradient centrifugation, the different components of the cell can be separated based on their size, density, or solubility.
Keeping the tissue sample in an isotonic solution prior to homogenization is crucial for maintaining the osmotic balance of the cells. An isotonic solution has the same concentration of solutes as the cells, which prevents the cells from shrinking or swelling due to osmotic changes. By keeping the tissue sample in an isotonic solution, the physiological conditions within the cells are maintained, ensuring that the cellular components remain intact and undamaged during the subsequent homogenization process.
Additionally, refrigerating the tissue sample helps to slow down metabolic activities and enzyme degradation that can occur at higher temperatures. By keeping the tissue sample in a cold environment, the breakdown of cellular components due to enzymatic activities is minimized, ensuring better preservation of the cellular structures.
In summary, keeping the tissue sample in an isotonic solution in a refrigerator prior to homogenization in cell fractionation helps maintain the structural integrity and physiological conditions of the cells, ensuring accurate and reliable separation of cellular components during subsequent steps of the fractionation process.