posted by Matthew .
A stock protein sample that has a concentration of 250.0mg/ml. You need to dilute this sample to 20.0 mg/ml in buffer. The final volume of the diluted solution is 5.0ml. how much of the stock protein solution and buffer are required to achieve this? Answer with appropriate sig figs.
you want 5ml of 20mg/ml in buffer.
you are diluting the solution 250/20 times, or 12.5 times. So you need one part original protein sample, 11.5 parts buffer.
What is each part? 1 part=5.0ml/12.5=4ml
11.5 parts= 46ml