Five ul of a 10 to 1 dilution sample was added to 3ml of Bradford reagent. The absorbable reading was 0.68. According to the standard curve this represents 0.05mg of protein. Determine the stock concentration of the protein solution in mg/ml
To determine the stock concentration of the protein solution in mg/ml, we need to calculate the concentration of the diluted protein solution first.
First, let's calculate the concentration of the diluted protein solution in mg/ml:
- The absorbance reading is 0.68.
- According to the standard curve, this corresponds to 0.05 mg of protein.
- Therefore, the concentration of the diluted protein solution is (0.05 mg) / (3 ml) = 0.0167 mg/ml.
Now, let's calculate the concentration of the stock protein solution in mg/ml:
- The diluted protein solution was prepared by adding 5 ul of the stock protein solution to 3 ml of Bradford reagent.
- The dilution factor is (3 ml) / (5 ul) = 600.
- Therefore, the concentration of the stock protein solution is 0.0167 mg/ml * 600 = 10 mg/ml.
To determine the stock concentration of the protein solution, we can use the formula:
Stock concentration (mg/ml) = (Absorbance / Protein amount) x Dilution factor
In this case,
Absorbance = 0.68
Protein amount = 0.05 mg
Dilution factor = 10 (10 to 1 dilution)
Let's calculate the stock concentration:
Stock concentration = (0.68 / 0.05) x 10 = 13.6 mg/ml
Therefore, the stock concentration of the protein solution is 13.6 mg/ml.
To determine the stock concentration of the protein solution in mg/ml, we need to go through the following steps:
Step 1: Calculate the amount of protein in the diluted sample
The concentration of the diluted sample can be determined by multiplying the absorbance reading by the amount of protein represented by that absorbance value according to the standard curve.
0.68 absorbance * 0.05 mg protein = 0.034 mg of protein in the diluted sample
Step 2: Calculate the amount of protein in the undiluted sample
To determine the amount of protein in the undiluted sample, we need to consider the dilution factor. In this case, 10 to 1 dilution means that the diluted sample is 1/10th of the concentration of the undiluted sample.
Therefore, to calculate the amount of protein in the undiluted sample, we need to multiply the amount of protein in the diluted sample by the dilution factor.
0.034 mg protein * 10 = 0.34 mg of protein in the undiluted sample
Step 3: Calculate the volume of the undiluted sample
We know that 5 μl of the diluted sample was used, so to calculate the volume of the undiluted sample, we need to divide the amount of protein in the undiluted sample by the concentration (stock concentration) of the protein solution.
0.34 mg protein / stock concentration = volume of the undiluted sample
Now, we have all the information we need to determine the stock concentration.
Please provide the volume of the undiluted sample to complete the calculation.