How do i plot i schild plot with this data:
Conc (M) 10^-8 Ec50= (microM) 12
To plot an IC50 (inverse concentration - response) or Schild plot with the given data, you will need additional data points. The data you have provided consists of a single concentration (10^-8 M) and its corresponding response value (EC50 = 12 µM).
A typical Schild plot requires a range of concentration values to determine the IC50, as it represents the concentration at which the response is half-maximal. Therefore, you will need at least two more concentration - response data points to construct the plot accurately.
To generate the Schild plot, you need to perform an additional assay using different concentrations of the substance and measure their corresponding responses. The concentration range should include values above and below the expected IC50 value. Aim for a logarithmic scale to evenly distribute the range of concentrations.
Once you have obtained data for at least three concentrations and their corresponding responses, follow these steps to plot the Schild plot:
1. Start by converting the concentration values into the appropriate logarithmic scale, usually by taking the negative logarithm of the base 10 of the concentration values (e.g., log10(concentration)).
2. Plot the logarithmic concentrations on the x-axis (independent variable) and the corresponding response values on the y-axis (dependent variable).
3. Fit a sigmoidal curve (such as a logistic or Hill equation) to the data points.
4. Determine the concentration at which the response is half-maximal (IC50) using the fitted curve or by directly finding the point where the response is half-maximal.
5. Calculate the logarithm of the IC50 value and plot it on a separate y-axis (log10(IC50)) next to the original y-axis.
6. Connect the data points using a line, including the IC50 value.
7. Calculate the slope of the line connecting the data points (usually through linear regression) and interpret its value in relation to the Schild equation.
8. It is also possible to calculate the IC50 value directly from the data points by interpolating between concentrations if the response is measured at sufficiently close intervals.
Keep in mind that obtaining reliable and accurate data is crucial for constructing an informative Schild plot.