A scientist receives a vial containing protein at a concentration of 35mg ml -1 and is asked to add 0.25ml of it to 6.75mls of Biuret solution. What is the concentration of protein in the Biuret solution in mkg/ml?
To find the concentration of protein in the final Biuret solution, we need to calculate the total amount of protein added and then divide it by the total volume of the final solution.
First, let's find the total amount of protein added to the Biuret solution:
The concentration of the protein in the vial is given as 35 mg/ml. We can convert this to µg/ml (micrograms per milliliter) by multiplying by 1000. Therefore, the concentration of the protein in the vial is 35,000 µg/ml.
Now, let's calculate the total amount of protein added:
The scientist is asked to add 0.25 ml of the protein from the vial to the Biuret solution. To find the total amount of protein added, we multiply the volume added by the concentration of protein in the vial:
Total protein added = 0.25 ml * 35,000 µg/ml = 8,750 µg
Next, we need to find the total volume of the final solution:
The scientist adds 0.25 ml of the protein to 6.75 ml of Biuret solution, so the total volume of the final solution is:
Total volume = 0.25 ml + 6.75 ml = 7 ml
Finally, we can calculate the concentration of protein in the Biuret solution by dividing the total protein added by the total volume of the final solution:
Concentration of protein in the Biuret solution = Total protein added / Total volume
Concentration of protein in the Biuret solution = 8,750 µg / 7 ml = 1,250 µg/ml
Therefore, the concentration of protein in the Biuret solution is 1,250 µg/ml.