We conducted an expirement to detect disulphide groups in protein. One tube(lysozyme) contained unkowns, had approx 9.34 micromoles of disulphide groups.
The question asks me to work backwards through all the dilution factors taken to give number of micromoles of disulphide groups per ml of original solution.
These are the steps taken to prepare the lysozyme tube:
- A tube of lysozyme solution of 1:32 dilution of lysozyme solution( 0.5mg/ml)
-0.0001M of FMA in 0.01M NAOH (provided) should be diluted 1:10 with 1M NaOH.
-Add 8ml of 1M NaOH
Please if you any further questions, let me know
thanks.
To work backwards and calculate the number of micromoles of disulfide groups per ml of the original solution, we need to go step by step through the dilution process.
Step 1: Calculate the concentration of the lysozyme solution used in the experiment.
The lysozyme solution has a 1:32 dilution, and the original concentration is given as 0.5mg/ml. To calculate the concentration of the lysozyme solution used in the experiment, we can multiply the original concentration by the dilution factor:
Concentration of lysozyme solution = 0.5 mg/ml x 1/32 = 0.015625 mg/ml
Step 2: Calculate the number of micromoles of disulfide groups in the lysozyme tube.
The lysozyme tube contains approximately 9.34 micromoles of disulfide groups.
Step 3: Calculate the volume of the lysozyme tube.
To calculate the volume of the lysozyme tube, we need to know how much volume was added to the tube during the dilution process. From the information provided, we can see that 8ml of 1M NaOH was added to the lysozyme tube.
Step 4: Calculate the dilution factor.
To calculate the dilution factor, we need to know the volume of the original solution used to prepare the lysozyme tube. Since this information is not provided, we cannot calculate the exact dilution factor.
Step 5: Calculate the number of micromoles of disulfide groups per ml of the original solution.
To calculate the number of micromoles of disulfide groups per ml of the original solution, we need to divide the number of micromoles in the lysozyme tube by the volume of the original solution used. Since we don't have the exact dilution factor, we cannot calculate this value.
In summary, without the information about the volume of the original solution used, we cannot calculate the number of micromoles of disulfide groups per ml of the original solution.