Which of the following characteristic differentiate Eubacteria from Archaebacteria?

1. Circular nature of chromosome.
2. Absence of nuclear membrane.
3. Presence of 70S ribosomes.
4. Presence of murein in cell wall.

To determine which characteristics differentiate Eubacteria from Archaebacteria, we will go through each option and explain its relevance.

1. Circular nature of chromosome: Both Eubacteria and Archaebacteria have a circular chromosome. So this characteristic does not differentiate them.

2. Absence of nuclear membrane: Both Eubacteria and Archaebacteria lack a nucleus and, therefore, a nuclear membrane. So this characteristic does not differentiate them either.

3. Presence of 70S ribosomes: Both Eubacteria and Archaebacteria have 70S ribosomes. Hence, this characteristic does not help us differentiate between the two.

4. Presence of murein in the cell wall: Murein, also known as peptidoglycan, is a polymer present in the cell walls of Eubacteria. However, Archaebacteria lack murein in their cell walls. This characteristic is specific to Eubacteria and allows us to differentiate them from Archaebacteria.

Therefore, the characteristic that differentiates Eubacteria from Archaebacteria is the presence of murein in the cell wall.