what is the retention time (RT) of polar and nonpolar stuff?
Do you want actual times? It depends upon the kind of STUFF, as you put it, and on the kind of stationary phase being used.
thats what i want to know, not exact time but depending on the stationary phase.
The retention time (RT) in chromatography refers to the time it takes for a particular component of a mixture to travel through the chromatographic system and elute from the column. The RT of a substance is influenced by various factors, including the polarity of the substance itself and the polarity of the chromatographic system being used.
In general, polar substances tend to have shorter retention times, while nonpolar substances have longer retention times. This is because polar substances interact more strongly with the stationary phase in the column, causing them to be retained for a shorter period of time. On the other hand, nonpolar substances have weaker interactions with the stationary phase, leading to longer retention times.
To determine the specific retention times of polar and nonpolar substances, you would typically need to perform chromatographic experiments using specific chromatographic techniques like gas chromatography (GC) or liquid chromatography (LC). These experiments involve separating and analyzing the components of a mixture by passing it through a column filled with a stationary phase.
During the chromatographic separation, the elution time or retention time of each substance is measured by monitoring the detector response. By comparing the retention times of known polar and nonpolar substances, you can establish a reference or calibration curve, which can be used to identify and quantify these substances in unknown samples based on their retention times.
In summary, the determination of retention times for polar and nonpolar substances requires performing chromatographic experiments using appropriate techniques and comparing the results with known standards or calibration curves.