Suppose that a species of fish has split into two populations in a lake: one on the eastern side and one on the western side. How could you determine whether the two populations constitute different species?

A. Examine the populations for morphological, color, or other physical differences.

B. Examine the populations for different genetic markers and allele frequencies.

C. Attempt to breed members from the two populations in captivity.

D. Introduce members from one population into the other population's habitat to see if they interbreed.

A. Examine the populations for morphological, color, or other physical differences.

B. Examine the populations for different genetic markers and allele frequencies.

These two methods would provide valuable information about the genetic and physical differences between the two populations, which could help determine if they have diverged enough to be considered different species. The other two options are not as reliable for determining species differentiation.