Yes, Spider-Man and the Teenage Mutant Ninja Turtles have appeared together in several crossover comic books and animated TV series episodes. One notable example is the "Spider-Man/TMNT" crossover comic book series published by Marvel Comics and IDW Publishing in 2019. In this series, Spider-Man teams up with the TMNT to take on a group of villains threatening both New York City and the Turtles' home in the sewers.
