The sample size was not explicitly mentioned in the text provided. However, based on the description of the experimental setup, it appears that multiple Catharanthus roseus plants were used in the study to investigate the accumulation of lead and nickel over a period of 60 days.

While the specific number of plants used in the study is not mentioned, it is important to note that the sample size is a critical factor in determining the representativeness of the study findings for the larger population. A larger sample size generally leads to more reliable and generalizable results.
In research studies, the sample size should be determined based on statistical considerations and power analysis to ensure that the findings are valid and applicable to the broader population of interest. It is recommended to report the sample size and sampling method in scientific research articles to provide transparency and reproducibility of the study.
The sample size and specific details regarding it were not provided in the information reviewed. However, it is essential to have a sufficiently large and diverse sample size to ensure the results are representative of the larger population.
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The exact sample size used in the study on the accumulation of lead and nickel in Catharanthus roseus plants over a 60-day period was not specified, but it is crucial to have an adequate sample size for research findings to be reliable and applicable to the wider population. Reporting the sample size and sampling method in research articles is important for transparency and reproducibility of the study.