Dissolve 0.1gm of glucose in 2cc if water. To this dilute solution in a test tube, add 2 drops of molischs reagent and shake. Carefully pour 2cc o conc H2SO4 down the side of the tube and allow to stand for 2 minutes
State your observations
The exact observations would depend on the specific experiment and the concentration of the molischs reagent. However, some possible observations could be:
- The glucose dissolves in the water, resulting in a clear or slightly cloudy solution.
- When the molischs reagent is added and the test tube is shaken, a color change may occur depending on the reagent's concentration. This color change could range from brown to purple or violet, indicating the presence of carbohydrates.
- When the concentrated H2SO4 is carefully poured down the side of the tube, it may form a separate layer on top of the solution.
- After allowing the mixture to stand for 2 minutes, there may be further color changes or reaction occurring. This could include the formation of a ring or other appearance changes at the interface between the H2SO4 layer and the glucose solution.
Remember, these are just possible observations and the actual results may vary depending on the experimental conditions. It is always important to conduct experiments carefully and accurately to obtain reliable results.