can you get the amoeba proteus in sharp focus using the high-power objective? explain your answer. why or why not?
No, it is not possible to get the Amoeba proteus in sharp focus using the high-power objective.
The high-power objective has a higher magnification, typically around 40x or 100x, which allows for more detailed examination of the specimen. However, it also has a shorter working distance and a smaller depth of field compared to lower magnification objectives.
Amoeba proteus is a relatively large and constantly changing organism that moves by extending and retracting its pseudopods. Due to its size and constant motion, it becomes difficult to keep the amoeba within the limited depth of field provided by the high-power objective. As a result, the focus will often be lost, and it becomes challenging to get a clear, sharp image of the Amoeba proteus using the high-power objective.
To observe Amoeba proteus in sharp focus, the low-power or medium-power objectives with a wider depth of field would be more suitable. These objectives offer a greater working distance, allowing for more flexibility in focusing on the organism's constantly changing shapes and movements.