A scientist wants to study the cytochrome c gene of a fruit fly. The DNA for the gene is extracted and subjected to several treatments.Question 1Use the passage to answer the next four questions.Part A: During cloning, the DNA is cut with a restriction enzyme giving it what?(1 point)Responsesmore intronsmore intronssticky endssticky ends a polyA tail a polyA taila binding site for a transcription factora binding site for a transcription factorQuestion 2Part B:If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use?(1 point)ResponsesPCRPCRepigeneticsepigeneticshybridizationhybridizationgel electrophoresisgel electrophoresisQuestion 3Part C:If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? (1 point)Responsestranscription factortranscription factorrRNArRNAribosomeribosomeRNA polymeraseRNA polymeraseQuestion 4Part D:If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what?(1 point)ResponsesPCRPCRgene therapygene therapystem cell workstem cell workDNA fingerprintingDNA fingerprintingSkip to navigation
Question 1: The DNA is cut with a restriction enzyme giving it sticky ends.
Question 2: The scientist would use PCR (Polymerase Chain Reaction) to have a large number of copies of the gene.
Question 3: Different levels of gene expression in different cells of the same organism would be responsible for transcription factors.
Question 4: If a disease were identified as being caused by defects in the cytochrome c gene, the isolated copy could be used for gene therapy.