Directions: Revise the figure legends to improve clarity in each section. Then provide explanation of each in short and clear paragraphs for each one below.
1. Predicted domains of proteins encoded by newly identified genes.
Novel = no known function, TM = putative transmembrane domain, PB = putative protein binding domain, kinase = putative kinase activity.
2. Phenotypes of mutants recovered from F3 mutagenic screen. (A) Mutagenesis and genetic screen strategy. (B) wild type (C) no eye mutant (D) little eye 1 mutant (E) little eye 2 mutant (F) ley1 ley2 double mutant (G) ney ley1 double mutant (H) ney ley2 double mutant.
3. Correlations between mutant and wild type larval transcript levels. In each panel a dot is color-coded probes to match the gene on x or y axis. The blue arrow indicates an unknown transcript (JY.8675309).
4. Subcellular localization of JY.867 in cultured cell line. A) Background fluorescence in untransfected cells. B) Cells transfected with expression plasmid encoding JY.8675309. C) Cells cotransfected with Ley2 and JY.8675309 expression plasmids. D) Cells cotransfected with expression plasmids encoding Ley2, JY.8675309, and short hairpin specific to Ley1 mRNA.
5. Phenotypes resulting from siRNA treatment of larval proto-eyes. A) Typical phenotype resulting from embryo injection with scrambled siRNAs. B) Phenotype resulting from injection of ney siRNA. C) Phenotype resulting from injection of siRNA specific to novel gene JY.8675309.
1. Predicted domains of proteins encoded by newly identified genes:
The figure legend describes the predicted domains of proteins encoded by newly identified genes. It indicates that the novel genes have no known function, but they have putative transmembrane domains (TM), putative protein binding domains (PB), or putative kinase activity. This information helps to categorize and understand the potential functions of these newly identified genes.
2. Phenotypes of mutants recovered from F3 mutagenic screen:
The figure legend explains the different phenotypes observed in mutants recovered from an F3 mutagenic screen. It includes several panels designated by letters (A-H). Panel A describes the mutagenesis and genetic screen strategy. Panels B-H represent different mutant phenotypes, including wild type, no eye mutant, little eye 1 mutant, little eye 2 mutant, ley1 ley2 double mutant, ney ley1 double mutant, and ney ley2 double mutant. These descriptions provide a clear understanding of the various mutant phenotypes observed in the study.
3. Correlations between mutant and wild type larval transcript levels:
The figure legend describes the correlations between mutant and wild type larval transcript levels. Each panel in the figure represents a specific correlation. The dots in the panel are color-coded probes that match the gene on the x or y axis. Additionally, there is a blue arrow indicating an unknown transcript labeled JY.8675309. This legend provides clarity on how the correlations between transcript levels are represented and highlights the presence of an unknown transcript.
4. Subcellular localization of JY.867 in cultured cell line:
The figure legend explains the subcellular localization of JY.867 in a cultured cell line. Panel A depicts the background fluorescence in untransfected cells as a reference. Panel B represents cells transfected with an expression plasmid encoding JY.8675309, showing the localization of the protein. Panel C shows cells cotransfected with Ley2 and JY.8675309 expression plasmids, indicating any potential co-localization between the two proteins. Panel D illustrates cells cotransfected with expression plasmids encoding Ley2, JY.8675309, and a short hairpin specific to Ley1 mRNA, providing insights into the effect of Ley1 knockdown on the subcellular localization of JY.8675309. This legend clearly explains the different experiments and provides an understanding of the subcellular localization patterns observed.
5. Phenotypes resulting from siRNA treatment of larval proto-eyes:
The figure legend describes the phenotypes resulting from siRNA treatment of larval proto-eyes. Panel A shows the typical phenotype observed when scrambled siRNAs are injected into the embryos. Panel B represents the phenotype resulting from the injection of ney siRNA. Panel C illustrates the phenotype resulting from the injection of siRNA specific to the novel gene JY.8675309. This legend provides a concise summary of the different phenotypes observed as a result of siRNA treatments on larval proto-eyes.