How did Spain's refusal to destroy Negro Fort affect Florida?

(1 point)
It led to General Jackson's troops' first invasion of the territory.
It gave the Seminoles an empowered stance against the United States.
It showed that Spain did not view General Jackson as a significant threat.
It proved that Spain was not going to give up the territory without a bitter fight.

All of the given options contain some truth, but the most accurate answer is:

It led to General Jackson's troops' first invasion of the territory.