You want to create a 1:20 dilution of a bacterial culture. You want a total of 40 ml of diluted bacterial culture, how much culture would you add to how much media?
add 1 ml culture to 20 ml media
add 2 ml culture to 40 ml media
add 2 ml culture to 38 ml media
add 2 ml culture to 42 ml media
The correct option would be to add 2 ml of culture to 38 ml of media in order to create a 1:20 dilution of a bacterial culture with a total volume of 40 ml.
To create a 1:20 dilution of a bacterial culture with a total volume of 40 ml, you would add 2 ml of the culture to 38 ml of media.
To create a 1:20 dilution of a bacterial culture, you need to add 1 part of the culture to 20 parts of media. In this case, you want a total of 40 ml of diluted bacterial culture.
The correct option would be to add 2 ml of culture to 40 ml of media.
This calculation is based on the ratio of 1 part culture to 20 parts media. If you have 2 ml of culture, you need to add 2 x 20 = 40 ml of media to maintain this dilution ratio. So, the correct answer is to add 2 ml of culture to 40 ml of media.