In Macbeth, the “damned spot” to which Lady Macbeth refers is

(1 point)
Duncan’s bloodstains.
the castle.
Birnam Wood.
Macbeth.

Duncan’s bloodstains.

The "damned spot" to which Lady Macbeth refers in Macbeth is Duncan's bloodstains.

To determine the answer to this question, we need to analyze the play Macbeth by William Shakespeare. Lady Macbeth mentions a "damned spot" within the play, and we need to identify what this spot refers to.

In Act V, Scene 1 of Macbeth, Lady Macbeth is sleepwalking and trying to wash an invisible stain off her hands, exclaiming, "Out, damned spot! Out, I say!" This scene reveals Lady Macbeth's guilt and remorse for the act of murder committed by Macbeth and herself.

Based on this information, we can conclude that the "damned spot" refers to the bloodstains from Duncan's murder. Therefore, the correct answer is "Duncan's bloodstains."