why is aspetic technique used to transfer bacteria?
a. to get a variety of different microorganisms in the culture.
b. to transfer only wanted bacteria onto petri dish
c. to get as many pathogens as possible in petri dish.
The correct answer is "b. to transfer only wanted bacteria onto the petri dish."
Aseptic technique is used to prevent contamination during the transfer of bacteria. Contamination can arise from unwanted microorganisms that may be present in the environment or on the equipment being used. By employing aseptic technique, the transfer of only the desired microorganisms can be ensured. This is important in microbiology and other scientific applications where specific bacteria need to be isolated and studied.
The aseptic technique is used to transfer bacteria to minimize the introduction of contaminants and ensure the isolation and pure cultivation of the desired bacteria. The correct answer is option (b) - to transfer only the wanted bacteria onto a petri dish.
By using aseptic technique, the risk of introducing unwanted microorganisms is reduced, thereby ensuring the accuracy and purity of the culture. The goal is to prevent cross-contamination and maintain a controlled environment to study, identify, or manipulate specific bacteria.
The correct answer to the question is b. to transfer only wanted bacteria onto a petri dish.
Aseptic technique is a set of practices used to prevent contamination of cultures, samples, or environments by unwanted microorganisms. It is essential in microbiology and other laboratory settings to maintain sterile conditions and ensure accurate and reliable results.
When transferring bacteria, the goal is typically to isolate and grow a pure culture of a specific bacterium for further study or identification. Aseptic technique helps achieve this by minimizing the introduction of unwanted bacteria or other contaminants.
To transfer bacteria using aseptic technique, the following steps are typically followed:
1. Prepare a clean, sterile work area: Use a laminar flow hood or a clean bench, ensuring that it has been properly decontaminated and sterilized.
2. Disinfect the surfaces and instruments: Wipe down the work area and any tools or equipment with a suitable disinfectant, such as ethanol or a bleach solution, to eliminate any potential contaminants.
3. Flame sterilization: Using a Bunsen burner or other suitable heat source, carefully flame sterilize any metal tools (e.g., inoculating loop) by passing them through the flame until they turn red-hot. This kills any bacteria present on the surface of the tool.
4. Inoculation: Using the sterilized tools, transfer a small sample of the desired bacteria onto the petri dish containing a suitable growth medium. It is important to avoid touching any non-sterile surfaces or allowing the tools to come into contact with the open air to prevent contamination.
5. Incubation: Once the bacteria have been transferred, the petri dish is sealed with tape or a lid and placed in an incubator set at the appropriate temperature and conditions for the bacteria to grow.
By employing proper aseptic technique, the risk of introducing unwanted microorganisms into the bacterial culture is minimized, allowing for the isolation and growth of the specific bacteria of interest.