in conservative DNA replication is both formed DNA is newly synthesized
I'm sorry, but I'm not sure I understand your question. Are you asking about the process of DNA replication in conservative DNA?
In conservative DNA replication, the newly formed DNA is not synthesized, but rather a copy of the original DNA molecule is made.
To understand the concept of conservative DNA replication, let's first briefly explain the process of DNA replication. DNA replication is the process by which a cell duplicates its DNA prior to cell division. It ensures that each daughter cell receives an exact copy of the genetic material.
During DNA replication, the double-stranded DNA molecule unwinds, and the two strands separate. Each separated strand then serves as a template for the synthesis of a new complementary strand. This results in the formation of two identical DNA molecules, each with one original strand and one newly synthesized strand.
In conservative DNA replication, one of the two DNA helices is preserved as the original molecule, while the other helix serves as a template for the synthesis of an entirely new DNA molecule. This means that after replication, one double-helix contains both the original strands (parental DNA), and the other double-helix consists entirely of newly synthesized strands (daughter DNA).
To determine which DNA replication mechanism is occurring, scientists have used isotopic labeling experiments and employed techniques such as density gradient centrifugation. These methods provide insight into the distribution of heavy isotopes among the newly synthesized DNA strands.
In conclusion, in conservative DNA replication, one DNA molecule is preserved in its original form, while the other DNA molecule is entirely newly synthesized.
In conservative DNA replication, a new DNA molecule is synthesized while the original DNA molecule remains intact. The process involves the following steps:
1. Initiation: The replication process begins at specific sites on the DNA molecule called origins of replication. Proteins called initiator proteins bind to these sites, creating a replication bubble.
2. Unwinding: Enzymes called helicases unwind the double-stranded DNA molecule by breaking the hydrogen bonds between the complementary base pairs. This creates a replication fork, where the two strands separate and become accessible for the replication process.
3. Priming: Enzymes called primases synthesize RNA primers at the start of each DNA strand. These primers provide a free 3' OH group for DNA polymerase to begin adding nucleotides.
4. Elongation: DNA polymerase adds nucleotides to the growing DNA strands in a 5' to 3' direction. The leading strand is synthesized continuously in the same direction as the replication fork, while the lagging strand is synthesized in short fragments called Okazaki fragments.
5. Proofreading and Repair: DNA polymerase has a proofreading function, which helps to correct any errors that may occur during replication. Additionally, various repair enzymes are involved in correcting any mistakes that go undetected by the proofreading process.
6. Termination: The replication process continues until the entire DNA molecule has been copied. In conservative replication, the newly synthesized DNA strands are hybridized with the original DNA strands, resulting in two molecules of double-stranded DNA.