tetracycline is an antibiotic that blocks trna from entering the ribosome and associating with mrna. if tetracycline were added to the cytoplasm of a eukaryotic cell, which types of proteins would be made?
a. only proteins that function in the cytoplasm of the cell.
b. only proteins that will be exported outside of the cell.
c. no proteins will be made if trna cannot bind to mrna
d. only proteins that will be inserted into a membrane
The correct answer is a. Only proteins that function in the cytoplasm of the cell.
Tetracycline inhibits the binding of tRNA to the ribosome and prevents the association with mRNA, which is essential for protein synthesis. Since eukaryotic cells have compartmentalization and different protein synthesis machinery in different cellular regions, the absence of tRNA-mRNA association in the cytoplasm would only affect the synthesis of cytoplasmic proteins. Proteins that are targeted for export outside of the cell or insertion into membranes have different mechanisms in place, such as signal sequences and specific targeting factors, which are not affected by tetracycline.
If tetracycline is added to the cytoplasm of a eukaryotic cell, the types of proteins that would be made are:
c. no proteins will be made if tRNA cannot bind to mRNA
This is because tetracycline blocks tRNA from entering the ribosome and associating with mRNA. Since protein synthesis relies on the binding of tRNA to mRNA during the process of translation, the inability of tRNA to bind to mRNA would prevent the synthesis of any proteins in the cell.
To determine which types of proteins would be made if tetracycline were added to the cytoplasm of a eukaryotic cell, we need to understand how tetracycline affects protein synthesis.
Tetracycline is an antibiotic that specifically blocks tRNA from entering the ribosome and associating with mRNA. This means that it prevents the translation process by inhibiting the binding of tRNA to mRNA during protein synthesis.
In eukaryotic cells, protein synthesis occurs in different locations within the cell. The cytoplasm is where many proteins are synthesized for functions within the cell, while proteins destined for export or insertion into the membrane require additional steps.
Based on this information, the correct answer is (a) only proteins that function in the cytoplasm of the cell. Since tetracycline blocks the binding of tRNA to mRNA, it would primarily affect the proteins being synthesized in the cytoplasm. Therefore, proteins that are meant to function within the cytoplasm of the cell would still be produced.
Proteins that are exported outside of the cell or inserted into the membrane require additional processing steps, such as signal sequences or post-translational modifications. These steps involve specific mRNA-tRNA interactions that could be disrupted by tetracycline, resulting in a lack of production for those types of proteins.
It's important to note that tetracycline's effect on protein synthesis can vary depending on various factors, such as concentration and specific cell type. This answer assumes a general understanding of its mechanism of action.