In 1859, Louis Pasteur designed an experiment to address spontaneous generation. He used a curved-neck flask that allowed the air inside the flask to mix with air outside the flask, but prevented solid particles, like microorganisms, from entering the body of the flask. Broth boiled inside the experimental curved neck flasks remained clear for up to a year. But when Pasteur broke off the curved

necks, the broth became cloudy and contaminated within a day. Pasteur reasoned that the contamination was due to microorganisms in the air.

Based on the results of Pasteur's experiment, formulate a conclusion regarding the expected results in the third flask?
Responses
A No microorganisms will grow in the broth because the broth has been boiled to kill all the cells.No microorganisms will grow in the broth because the broth has been boiled to kill all the cells.
B No microorganisms will grow in the broth because the microorganisms are only in the air, not in the broth.No microorganisms will grow in the broth because the microorganisms are only in the air, not in the broth.
C Microorganisms will grow in the broth because tipping the flask exposes the broth to microorganisms in the air.Microorganisms will grow in the broth because tipping the flask exposes the broth to microorganisms in the air.
D Microorganisms will grow in the broth because they already exist in the broth. Boiling doesn't destroy all of the microorganisms.

C Microorganisms will grow in the broth because tipping the flask exposes the broth to microorganisms in the air.

The correct response is:

C. Microorganisms will grow in the broth because tipping the flask exposes the broth to microorganisms in the air.