Speciation can be caused by:

Isolation due to a natural disaster like tornado
Mating at different times of the year
Eating different types of food
all of these

options are possible causes of speciation, although they may not necessarily lead to speciation in all cases. Other potential causes include geographic isolation, changes in habitat or environmental conditions, genetic mutations, and selective pressures from predators or other factors.

Yes, all of these factors can potentially contribute to speciation. Speciation refers to the process by which new biological species arise. It can occur when a population of organisms becomes isolated in some way, preventing gene flow with other populations of the same species. This isolation can be caused by various factors, including natural disasters like tornadoes that physically separate populations. Additionally, differences in mating times or preferences can lead to reproductive isolation, where individuals from different populations are no longer able to successfully mate and produce fertile offspring. Similarly, differences in food sources can also contribute to speciation by driving adaptations in organisms that eventually lead to the formation of distinct species.