The gene frequency for a particular trait in a population in Texas was determined to be 80% X (dominant) and 20% x (recessive allele). Twenty years later, the gene frequency was determined to be 65% X and 35% x. What does this change indicate about the gene pool?

It has remained stable.
It lacks nutrition.
It is evolving.
It has become predominantly recessive.

It is evolving.

The change in gene frequency from 80% X and 20% x to 65% X and 35% x indicates that the gene pool is evolving. This change suggests a shift in the proportion of individuals carrying the dominant and recessive alleles over time.