Using the data below calculate the unknown concentration in mg/ml
Protein (ug) A
15 0.07
25 0.15
50 0.28
100 0.55
150 0.90
Unknown concentration = (150 x 0.90) / 15 = 12 mg/ml
To calculate the unknown concentration in mg/ml, we need to find the equation relating the protein concentration in ug to the concentration in mg/ml.
The equation is as follows:
Concentration (mg/ml) = Protein (ug) x Conversion Factor
To find the conversion factor, we need to determine the concentration in mg/ml for one of the protein measurements in the given data.
Let's choose the first data point: Protein (ug) = 15, Concentration (mg/ml) = 0.07.
So, the conversion factor is calculated as:
Conversion Factor = Concentration (mg/ml) / Protein (ug)
= 0.07 mg/ml / 15 ug
= 0.00467 mg/ug
Now, we can determine the unknown concentration in mg/ml by multiplying the protein measurement (ug) by the conversion factor.
For example, let's calculate the unknown concentration for a protein measurement of 25 ug:
Unknown Concentration = Protein (ug) x Conversion Factor
= 25 ug x 0.00467 mg/ug
= 0.11675 mg/ml
Similarly, you can calculate the unknown concentration for the remaining protein measurements using the same formula.
To calculate the unknown concentration in mg/ml, we can use the given data points to create a standard curve and then use this curve to determine the concentration of the unknown sample.
First, let's convert the given protein values from micrograms (ug) to milligrams (mg) by dividing each value by 1000.
Protein (mg) A
0.015 0.07
0.025 0.15
0.050 0.28
0.100 0.55
0.150 0.90
Next, we need to plot the data points on a graph with the protein concentration (mg/ml) on the x-axis and the corresponding absorbance (A) on the y-axis. This will create a standard curve.
Once the data points are plotted, we can use a trendline or fit a linear regression line to determine the equation of the line. The equation should be in the form of y = mx + b, where y is the absorbance (A), x is the protein concentration (mg/ml), m is the slope, and b is the y-intercept.
Using this equation, we can substitute the absorbance value of the unknown concentration into the equation to calculate the corresponding protein concentration (mg/ml).