List factor affect enzyme activity?

1. Temperature: Enzymes are sensitive to temperature and will become denatured if the temperature is too high or too low.

2. pH: Enzymes have an optimal pH range in which they work best.

3. Substrate concentration: Increasing the substrate concentration can increase the rate of reaction.

4. Enzyme concentration: Increasing the enzyme concentration can increase the rate of reaction.

5. Cofactors: Some enzymes require cofactors such as metal ions or vitamins to function properly.

6. Inhibitors: Inhibitors can bind to enzymes and prevent them from working properly.