The health, management and conservation of biodiversity, is a challenge facing ecosystems worldwide. The major factors that affect the health and function of the Great Barrier Reef are climate change and pollution. Which question investigates the effects of a biotic factor on the reef ecosystem?

To investigate the effects of a biotic factor on the Great Barrier Reef ecosystem, the following question can be asked:

1. How does the presence or absence of a particular species impact the health and functioning of the Great Barrier Reef ecosystem?

To answer this question, you would need to gather data and conduct research on the specific species in question and its interactions with other organisms within the reef ecosystem. This may involve examining the species' role in nutrient cycling, predator-prey dynamics, competition for resources, and symbiotic relationships. By analyzing data and researching relevant scientific studies, you can understand the biotic factors and their effects on the reef ecosystem.