Grade 11 learners want to investigate which enzyme is responsible for chemical digestion of starch into glucose in humans.They are provided with two different enzymes ( carbohydrase and protease),starch powder,warm water at about 37 degrees Celsius and basic apparatus and chemicals to test for glucose .
1.1 select and arrange the method steps in the correct sequence from the list below:
-allow apparatus to stand for an hour
-make two solutions of the same amount of starch,with warm water in test tube 1 and 2
-add 5ml carbohydrase to test tube 1 and 5ml protease to test tube 2
-Place the test tubes in a dark cupboard.
-Add Benedict's solution or Fehling's A +B,in the test tubes to test for glucose.
-Place the test tubes in a warm bath.
-if the contents change from light blue to green /yellow /orange in the test tube,then the enzyme has digested starch to glucose and the hypothesis must be accepted for the chosen enzyme.
-Make two solutions of different amounts of starch ,with warm water in test tube 1 and 2.
-If the contents remain light blue in the test tube ,then the enzyme has not digested starch to glucose and the hypothesis must be rejected for the chosen enzyme.
guyz help
Something
The correct sequence for the method steps is as follows:
1. Make two solutions of the same amount of starch, with warm water in test tube 1 and test tube 2.
2. Add 5ml of carbohydrase to test tube 1 and 5ml of protease to test tube 2.
3. Place the test tubes in a warm bath.
4. Allow the apparatus to stand for an hour.
5. Place the test tubes in a dark cupboard.
6. Add Benedict's solution or Fehling's A + B to the test tubes to test for glucose.
7. If the contents change from light blue to green/yellow/orange in the test tube, then the enzyme has digested starch to glucose, and the hypothesis for the chosen enzyme must be accepted.
8. If the contents remain light blue in the test tube, then the enzyme has not digested starch to glucose, and the hypothesis for the chosen enzyme must be rejected.