The velocity of an enzyme catalyzed reaction was 10% of Vmax at 2.0 μM substrate concentration. What was the Km in μM?

I know Km=1/2Vmax but I do not know if this is the right equation to use or if I am incorporating the known substrate concentration right?
I tried to set up the equation so
2uM= 1/2(1/10*Vmax)
and got v max= 40
then I took the same equation km=1/2vmax and plugged in the vmax of 40 to get a km concentration of 20uM. But the correct answer is 18uM.

Thannk you.

  1. 👍
  2. 👎
  3. 👁
  1. Use
    v0= vmax*[S]/(km+[S])
    where v0= 1/10*vmax
    km= 1/2vmax

    plug and chug, solve for vmax and plug into km=1/2vmax equation to get km

    1. 👍
    2. 👎

Respond to this Question

First Name

Your Response

Similar Questions

  1. science

    Q.1 Cellular respiration is an exothermic reaction and photosynthesis is an endothermic reaction. How does the required activation energy compare for these two reactions? 1. Cellular respiration needs a smaller amount of energy to

  2. This is grade 12 biology

    What is the effect of enzyme concentration on enzyme activity? Explain how much enzyme activity changes as enzyme concentration decreases, and discuss why this occurs (on a molecular level). Is this right (and can you add more):

  3. Biology

    What are the roles of hydrogen peroxide, oxygen, hydrogen, and catalase in the following chemical reaction? catalase 2H2O2 > O2 + 2H2O A. Catalase is an enzyme, O2 and H2O are substrates, and H2O2 is a reactant. B. Catalase is a

  4. Chemistry

    True or false? All enzymes are specific to a single substrate chemical. That is, an enzyme will catalyze reactions of one molecule only.

  1. Chemistry

    For any enzyme that follows simple Michaelis-Menten kinetics, when the initial Velocity of the reaction is 80% of Vmax what is the substrate concentration? The answer is: [S] = 4Km Can you explain why it is?

  2. science

    Question: what do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops?Predict what thte rate would be for 5 drops. Answer:Roughly, the rate doubles when the concentration of

  3. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of

  4. Chemistry

    At body temperature (37C), K of an enzyme-catalyzed reaction is 2.3x10^14 times greater than k of the uncatalyzed reaction. Assuming that the frequency factor A is the same for both reaction, by how much does the enzyme the E^a?

  1. chemistry...asap

    (c) Potassium hydroxide reacts completely with sulfuric acid according to the equation: H2SO4(aq) + 2KOH(aq) K2SO4(aq) + 2H2O(l) 0.95 g of potassium hydroxide were dissolved in water and made up to 250 cm3 of solution. 25 cm3

  2. Biology for school

    What is the effect of substrate concentration on enzyme activity? How does enzyme activity change as substrate concentration decreases? Explain on a molecular basis. I am not sure what the question wants from me. Would this be

  3. chemistry

    The activation energy of a certain uncatalyzed biochemical reaction is 50.0 kJ/mol. In the presence of a catalyst at 37°C, the rate constant for the reaction increases by a factor of 2.50 ✕ 103 as compared with the uncatalyzed

  4. chemistry

    . An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso

You can view more similar questions or ask a new question.