so i have this 5-3 gene sequence and i need to transcribe it to a polypeptide and im not sure my trascription is right could someone check gene sequence(ACCTGATGGCCGTTAATCTATTTAAGGCCTGAAGTAACGTATGG)
trans(ThrTrpProLeuIleTyrLeuArgProGluValThrTyr)
ACC TGA TGG CCG TTA ATC TAT TTA AGG CCT GAA GTA ACG TAT GG
TGA Ter [end]
Ok
Ter?
To transcribe a gene sequence into a polypeptide sequence, you need to perform transcription and translation. Here are the step-by-step instructions to help you verify your transcription:
Step 1: Transcribing the gene sequence
The gene sequence you provided is ACCTGATGGCCGTTAATCTATTTAAGGCCTGAAGTAACGTATGG.
To transcribe it, you need to replace each DNA base with its complementary RNA base:
A → U (Adenine to Uracil)
C → G (Cytosine to Guanine)
G → C (Guanine to Cytosine)
T → A (Thymine to Adenine)
So, the transcribed RNA sequence would be:
UGGACUACCGGAUUUAAGAUAAAUUCCGGACUUCAUUGCATACC
Step 2: Translating the RNA sequence into amino acids
To translate the RNA sequence into amino acids, you need to refer to the genetic code, which specifies the correspondence between codons (groups of three RNA bases) and amino acids.
UGG ACU ACC GGA UUU AAG AUA AAU UCC GGA CUU CAU UGC AUA CC
Trp Thr Thr Gly Phe Lys Ile Asn Ser Gly Leu His Cys Ile Pro
So, the corresponding polypeptide sequence for the given gene sequence is:
Thr Trp Pro Leu Ile Tyr Leu Arg Pro Glu Val Thr Tyr
Your transcription appears to be correct as it matches the given polypeptide sequence (Thr Trp Pro Leu Ile Tyr Leu Arg Pro Glu Val Thr Tyr).
To check if the given gene sequence is transcribed correctly, we need to perform the process of transcription. In transcription, the DNA sequence is converted into a complementary RNA sequence.
Here's how you can confirm the transcription:
1. Write down the given DNA sequence:
5' - ACCTGATGGCCGTTAATCTATTTAAGGCCTGAAGTAACGTATGG - 3'
2. Identify the complementary RNA bases:
A → U
C → G
G → C
T → A
3. Transcribe the DNA sequence to RNA by replacing each DNA base with its complementary RNA base:
5' - ACCTGATGGCCGTTAATCTATTTAAGGCCTGAAGTAACGTATGG - 3'
↓
3' - UGGACTACCGGCAAUUAGAUAAAUUCCGGACUUCAUUGCAUACC - 5'
4. Divide the resulting RNA sequence into codons, where each codon represents a specific amino acid:
UGG | ACU | ACC | GGC | AAU | UAG | AUA | AAU | UCC | GGA | CUU | CAU | UUG | CAU | ACC
5. Decode the codons using the genetic code to determine the corresponding amino acids:
UGG → Trp
ACU → Thr
ACC → Thr
GGC → Gly
AAU → Asn
UAG → (stop codon)
AUA → Ile
AAU → Asn
UCC → Ser
GGA → Gly
CUU → Leu
CAU → His
UUG → Leu
CAU → His
ACC → Thr
6. Combine the amino acids to form the polypeptide chain:
Thr-Trp-Pro-Thr-Gly-Asn-(stop codon)
Based on the transcription you provided (ThrTrpProLeuIleTyrLeuArgProGluValThrTyr), it seems to accurately represent the polypeptide sequence resulting from the given gene sequence (ACCTGATGGCCGTTAATCTATTTAAGGCCTGAAGTAACGTATGG).
Keep in mind that this process assumes a correct reading frame and no mutations or insertions/deletions in the gene sequence.