# Molecular cell Biology

The following chemicals have the indicated formula weights:Sucrose-342.3,Potassium dihydrogen phosphate(KH2PO40-136.09,Tris -121.14.You also have the following solutions made:20%SDS,5M NaCl,500mM EDTA.You have a 40%glycerol solution and 10X TBE.List the steps to make the following buffers.a)1 liter of a solution containing :0.5Msucrose,0.05M NaCl,0.02 M KH2PO4

1. 👍 0
2. 👎 0
3. 👁 121

## Similar Questions

1. ### Biology

The following chemicals have the indicated formula weight Surcose = 342.3g; Potassium dihydrogen=136.09; and Tris=121.14 Also the following solution already made: 20%SDS, 5M NaCl, 500mM EDTA. You have a 40% glycerol solution, and

asked by Robert on August 27, 2008
2. ### Biochemistry

How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1) and 1 M hydrochloric acid? I started with pH=pKa + log ([Tris]/[Tris+] [Tris]/[Tris+]= 10^(7.4-8.06]=0.219 so 0.219:1 ratio with

asked by Rose on January 5, 2011
3. ### chemistry

Prepare 1.5 liters of 0.25 M Tris buffer, pH 7.5. Useful information: pKa Tris = 8.1 Formula weight of Tris (hydroxymethyl aminomethane) base = 121.1 Formula weight of Tris hydrochloride (acid form) = 158.0

asked by steven on September 26, 2012

Calculate the weight of the buffers you will use to make the buffers for Part A for all the buffer possibilities listed under procedures in Part A, step 1. In other words, how many grams do you need to make 100 mL of a 0.1 M

asked by stew on January 27, 2008
5. ### Biochem

Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) necessary to make 100mL of a 0.100 M Tris buffer that is equimolar in Tris-base (MW=121.1 g/mol) and Tris-HCL (MW= 157.6g/mol)

asked by bugbabe623 on September 10, 2008
6. ### To Jessica

Sorry, but I made two errors. Monocalcium phosphate is monocalcium monohydrogen phosphate which is CaHPO4. The Ca is +2, H is +1, and PO4 is -3 so the +3 and -3 balance. And Bob Pursley did NOT suggest that name of monocalcium

asked by DrBob222 on December 16, 2007
7. ### Chemsitry

Tris is an abbreviation for tris (hydroxymethyl) aminomethane, which is a weak base. Calculate how many grams of tris (mw=121.14g/mol) are needed to make 25mL of 0.10M tris solution. Calculate how many mL of 0.25M HCl are needed

asked by Cassandra on April 2, 2012
8. ### biochemistry

how many grams each of tris and tris HCl are needed to make up 500ml of 1m tris buffer with a PH of 8.0. pka of tris =8.3 mwt of tris =121 mwt of tris HCl =175

asked by Anonymous on November 25, 2016
9. ### BCH

How many gramms of Tris and Tris Hcl are needed to make up 500ml of 1m Tris with a PH of 8.0? (pka of Tris is 8.3, mw of Tris is 121, mw of Hcl is 157.

asked by kishow on November 24, 2016
10. ### BIO

Can someone help with this question? Here are the formula weights for three compounds often used in molecular biology: NaCl, 58.44; Tris base, 121.1; EDTA, 372.2 a) For each of these three compounds, how much solute is required to

asked by Cindy on January 30, 2018

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