posted by S .
BSA stock solution A has a concentration of 3.50 mg/mL.
1.00mL of stock solution A is diluted to a final volume of 5.00mL to prepare solution B.
1.00mL of stock solution B is diluted to a final volume of 5.00mL to prepare solution C.
1.00mL of stock solution C is diluted to a final volume of 10.00mL to prepare solution D.
Calculate the concentrations of solutions B, C, and D.
A you are diluting it five times, meaning one part stock, four parts water. Five times means new B concentration is 3.50/5 mg/ml
diluting again five times. concentration of C is 3.5/25 mg/ml
dilting ten times. concentration D is 3.5/250 mg/ml