biochemistry

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The enzyme alcohol dehydrogenase catalyzes the following reaction:

Ethanol + NAD+  acetaldehyde + NADH + H+

You have a 1 ml solution containing 100 µM Ethanol, and 1 mM NAD+. You then add 1 mg of alcohol dehydrogenase.

Assume all of the ethanol gets converted to acetaldehyde.

a) What will the final concentration of NADH be in the solution?

b) What will the final absorbance of the solution be at 340 nm? (the molar extinction coefficient of NADH at 340 nm is 6.22 x 103 l/mol cm

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