chemistry lab

posted by .

An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the experiment? What is the final pH?

If needed: TRIS(Trizma base) mw= 121.1; pka= 8.3; TRIS-HCl mw= 157.6; pka= 8.3

I kind of have an idea. I start out with the hendersn hasselbalch equation and solve for the ratio ([Tris] / [Tris HCl]). I need help doing this first before i can proceed with the rest of the problem.

Thank you very much.

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS …
  2. chemistry

    This is my third time to ask this and no one has answered. Please help. An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result …
  3. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS …
  4. Biochemistry

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme esterase catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction?
  5. chemistry Please Help Dr.BOB

    Complete hydrolysis of 25 mM ethyl acetate in an enzyme (i.e., esterase)-catalyzed reaction is carried out in a 40 mM NaCl solution buffered by 0.2 M Tris-Cl, pH 9.1. What is the pH at the end of the reaction?
  6. Biology

    Okay so if i have two enzyme catalyzed reactions: 1) delta g of the ES = -22kJ/mol 2) delta g od the ES = 42 kJ/mol Is this first reaction going to have a higher reaction rate because the of the negative delta g, because doesnt a negative …
  7. Chemistry

    A reaction proceeds with ∆ H = 40 kJ/mol. The energy of activation of the uncatalyzed reaction is 120 kJ/mol, whereas i t is 55 kJ/mol for the catalyzed reaction. How many times faster is the catalyzed reaction than the uncatalyzed …
  8. Chemistry

    At body temperature (37C), K of an enzyme-catalyzed reaction is 2.3x10^14 times greater than k of the uncatalyzed reaction. Assuming that the frequency factor A is the same for both reaction, by how much does the enzyme the E^a?
  9. chemistry

    . An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso …
  10. Biochemistry

    An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso …

More Similar Questions