posted by alan .
An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the experiment? What is the final pH?
If needed: TRIS(Trizma base) mw= 121.1; pka= 8.3; TRIS-HCl mw= 157.6; pka= 8.3
I kind of have an idea. I start out with the hendersn hasselbalch equation and solve for the ratio ([Tris] / [Tris HCl]). I need help doing this first before i can proceed with the rest of the problem.
Thank you very much.