Chemistry

To determine v max and km of Beta-galactosidase for lactose, the same amount of enzyme (1 ug per tube) was incubated with a series of lactose concentrations under conditions where product concentrations remained negligible. At each lactose concentration, the initial reaction velocity was determined by assaying for the amount of lactose remaining at the end of the assay.

Question: Calculate 1/v and 1/[s] for each entry. (Please note the equations used.)

Lactose concentration (m/M) (believe is [s])
1
2
4
8
16
32
Rate of lactose consumption (u mol/min) (believe is v)

10.0
16.7
25.0
33.3
40.0
44.4

(Please show me how to do aleast the first two. And the formulas used.)

Question: When graphing is 1/v on the y-axis and 1/[s] on the y axis?

  1. 👍 0
  2. 👎 0
  3. 👁 90

Respond to this Question

First Name

Your Response

Similar Questions

  1. Science

    To determine v max and km of Beta-galactosidase for lactose, the same amount of enzyme (1 ug per tube) was incubated with a series of lactose concentrations under conditions where product concentrations remained negligible. At

    asked by Ahmad M. on September 15, 2013
  2. Chemistry

    To determine v max and km of Beta-galactosidase for lactose, the same amount of enzyme (1 ug per tube) was incubated with a series of lactose concentrations under conditions where product concentrations remained negligible. At

    asked by Ahmad M. on September 15, 2013
  3. chemistry help !!!!!!!!!!!!!!!!

    complete the following paragraph relating to the enzyme,b -galactosidase .this enzyme hydrolyses the disaccharide lactose to constituent monosaccharides ,galactose and glucose ,drag and drop the approprite words into the correct

    asked by john on February 2, 2013
  4. chemistry help !!!!!!!!!!!!!!!!

    complete the following paragraph relating to the enzyme,b -galactosidase .this enzyme hydrolyses the disaccharide lactose to constituent monosaccharides ,galactose and glucose ,drag and drop the approprite words into the correct

    asked by john on February 2, 2013
  5. biology help plz?????????????????

    Complete the following paragraph relating to the enzyme, β–galactosidase. This enzyme hydrolyses the disaccharide lactose to constituent monosaccharides, galactose and glucose. Drag and drop the appropriate words into the

    asked by centya on February 28, 2013
  6. chemistry

    β-Galactosidase (β-gal) is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. A 0.474 gram sample of β-galactosidase is dissolved in water to make 0.144 L of solution, and the osmotic

    asked by can i have some help? on August 29, 2017
  7. Biology

    Your research team suspects that lactose intolerance is a recessive trait. Which of the following observations would contradict your theory? a) Two lactose intolerant parents always have children who are lactose intolerant b)

    asked by Bella on January 17, 2011
  8. Biology Help Please!

    1 ) A temperature-sensitive mutation in the lacI gene of E. coli creates a repressor protein that has wild-type function at 30°, but is a superrepressor protein at 42° (unable to bind allolactose). You test this strain with and

    asked by Tenshi on April 9, 2016
  9. human physiology

    Please help, with the following question: "When an enzyme reaches its saturation point,..." A) the amount of substrate for the enzyme to act upon is very high B) the amount of substrate for the enzyme to act upon is very low C)

    asked by Michelle on August 27, 2013
  10. biology

    We recently performed a yeast fermentation lab in class to determine the effects of different sugars (sucrose, lactose and dextrose) on the rate of fermentation. I am writing up my lab report, however I am having some

    asked by Melissa on September 29, 2007

More Similar Questions