You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the desired buffer.
You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-EDTA (pH 8) and 2% SDS. You have plenty of the following stock solns.: 2M Tris-EDTA (pH 8) and 20% SDS. However, SDS cannot be autoclaved and must be added to the
How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1) and 1 M hydrochloric acid? I started with pH=pKa + log ([Tris]/[Tris+] [Tris]/[Tris+]= 10^(7.4-8.06]=0.219 so 0.219:1 ratio with
How much Tris-HCl would you need (in grams) to add to create 100 mL of a 0.223 M Tris buffer that is pH 9.64. The pKa of Tris-HCl is 8.30. I'm on my last try and thought I had it multiple times but keep getting it wrong.
what is the pH of the buffer when 4ml of 1M HCl is added to a 100ml buffer solution made using 5ml 1M Na2HPO4 and 5ml 1M NaH2PO4? The pKa for H+ + HPO4- ==> H2PO4 is 6.82. What would happen to the pH if the 100ml buffer had